Journal: Scientific Reports

Article Title: Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi

doi: 10.1038/s41598-021-03943-z

Figure Lengend Snippet: Male parent identification of A. coluzzii and A. stephensi offspring. Boxplots of relatedness values between each potential male parent and all offspring. Both species had a unique female parent and 4 potential male parents for A. coluzzii and 2 for A. stephensi . *** p -value < 0.0001; Wilcoxon rank-sum test.

Article Snippet: Anopheles coluzzii (MOPTI strain; MRA-763) and A. stephensi (STE2 strain; MRA-128) mosquito eggs were obtained from the Malaria Research and Reference Reagent Resource Center (BEI Resources, VA, USA) and reared at 26 °C and 80% relative humidity for multiple generations prior to experimental setup.

Techniques:

Journal: Scientific Reports

Article Title: Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi

doi: 10.1038/s41598-021-03943-z

Figure Lengend Snippet: Mendelian inheritance for male parent identification.

Article Snippet: Anopheles coluzzii (MOPTI strain; MRA-763) and A. stephensi (STE2 strain; MRA-128) mosquito eggs were obtained from the Malaria Research and Reference Reagent Resource Center (BEI Resources, VA, USA) and reared at 26 °C and 80% relative humidity for multiple generations prior to experimental setup.

Techniques:

Journal: Scientific Reports

Article Title: Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi

doi: 10.1038/s41598-021-03943-z

Figure Lengend Snippet: Candidate de novo mutations detected in A. coluzzii and A. stephensi by whole genome sequencing and validated by Sanger re-sequencing.

Article Snippet: Anopheles coluzzii (MOPTI strain; MRA-763) and A. stephensi (STE2 strain; MRA-128) mosquito eggs were obtained from the Malaria Research and Reference Reagent Resource Center (BEI Resources, VA, USA) and reared at 26 °C and 80% relative humidity for multiple generations prior to experimental setup.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi

doi: 10.1038/s41598-021-03943-z

Figure Lengend Snippet: Available direct estimates of mutation rate in vertebrates and invertebrates.

Article Snippet: Anopheles coluzzii (MOPTI strain; MRA-763) and A. stephensi (STE2 strain; MRA-128) mosquito eggs were obtained from the Malaria Research and Reference Reagent Resource Center (BEI Resources, VA, USA) and reared at 26 °C and 80% relative humidity for multiple generations prior to experimental setup.

Techniques: Mutagenesis

Journal: Scientific Reports

Article Title: Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi

doi: 10.1038/s41598-021-03943-z

Figure Lengend Snippet: Whole genome sequencing coverage results for each sample categorization.

Article Snippet: Anopheles coluzzii (MOPTI strain; MRA-763) and A. stephensi (STE2 strain; MRA-128) mosquito eggs were obtained from the Malaria Research and Reference Reagent Resource Center (BEI Resources, VA, USA) and reared at 26 °C and 80% relative humidity for multiple generations prior to experimental setup.

Techniques: Sequencing

Journal: Cell reports

Article Title: CD8 + T Cell Activation Leads to Constitutive Formation of Liver Tissue-Resident Memory T Cells that Seed a Large and Flexible Niche in the Liver.

doi: 10.1016/j.celrep.2018.08.094

Figure Lengend Snippet: Figure 3. Liver Trm Cells Derived from In Vitro-Activated Cells Patrol in the Liver Sinusoids 20 million in vitro-activated GFP-expressing OT-I cells were transferred into naive TdTomato mice that ubiquitously express a membrane form of TdTomato. Intravital 2-photon microscopy of the liver was performed 2–4 weeks after cell transfer. (A) Field of view of a 50-mm z stack. The scale bar represents 40 mm. See also Video S1. (B) Mean velocity of individual OT-I cells. A total of 10 movies from 5 mice were analyzed. Mean velocity ± SD is shown in red. (C) Single frames from intravital 2-photon imaging of GFP+ OT-I cells within the liver of TdTomato mice over time. The scale bar represents 10 mm. (D) Intravital 2-photon movies were quantitated for cells showing patrolling (crawling) behavior (black) or non-patrolling behavior (flowing in blood, sticking in sinusoids without crawling; white) (Fernandez-Ruiz et al., 2016). Mean ± SEM is shown, and a total of 10 movies from 5 mice were analyzed. (E) Memory T cells formed by activated OT-I cells protect mice from challenge with OVA-expressing sporozoites. Naive B6 mice were injected with 20 million activated OT-I cells or left untreated. 3 weeks after transfer, mice were challenged with 200 OVA-expressing PbA sporozoites and monitored for parasitemia. Mice were considered protected when they remained negative for parasites by day 12. Results are from 2 independent experiments using a total of 10 mice per group.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat monoclonal anti-CD8a (clone 53-6.7) Biolegend Cat#100748, RRID: AB_2562100 Rat monoclonal anti-CD8a (clone 53-6.7) BD Cat# 553035, RRID: AB_398527 Rat monoclonal anti-CD44 (clone IM7) Biolegend Cat# 103026, RRID: AB_493713 Rat monoclonal anti-CD62L (clone MEL-14) Biolegend Cat# 104432, RRID: AB_2285839 Armenian Hamster monoclonal anti-CD69 (clone H1.2F3) eBioscience Cat# 15-0691-82, RRID: AB_468772 Rat monoclonal anti-Va8.3 (clone B21.14) BD Cat# 553377, RRID: AB_394821 Rat monoclonal anti-Va2 (clone B20.1) BD Cat# 560624, RRID: AB_1727584 Parasite Strains Plasmodium berghei The Malaria Research and Reference Reagent Resource Center; BEI resources MRA-871 Plasmodium berghei ANKA-OVA Kimura et al., 2013 N/A Peptides and Recombinant Proteins SIINFEKL, SSIEFARL, KAVYNATC peptides Auspep N/A Recombinant human IL-2 Peprotech Cat# 200-02 Recombinant mouse IL-15 In vitro Technologies Cat# 447-ML Experimental Models: Mouse lines C57BL/6 Animal Resources Centre Stock # 000664 OT-I Hogquist et al., 1994 N/A gBT-I Mueller et al., 2002 N/A PbT-I Lau et al., 2014 N/A P14 Pircher et al., 1989 N/A Ly5.1/IL-15 / Kennedy et al., 2000 N/A Experimental models: Organisms/Strains Anopheles stephensi The Malaria Research and Reference Reagent Resource Center; BEI resources Strain: STE2/MRA-128 Recombinant DNA CpG 2006-21798 Geneworks Fernandez-Ruiz et al., 2016 CpG 2006-21798 Integrated DNA Technologies Fernandez-Ruiz et al., 2016 Software and Algorithms Imaris v9.1 Bitplane http://www.bitplane.com/ Prism v7 GraphPad https://www.graphpad.com/scientific- software/prism/ FlowJo v9.9 Tree Star https://www.flowjo.com/ Diva BD Bioscience N/A

Techniques: Derivative Assay, In Vitro, Expressing, Membrane, Microscopy, Imaging, Injection